Dr Boris Kysela

  • Position: Senior Lecturer in Human Molecular Genetics
  • Telephone: 0121 204 3631
  • Email: b.kysela@aston.ac.uk
  • Room Number: MB314B

A dedicated life-long student of genome stability and DNA repair fields.

  • 1980 – 1985  Charles University, Prague, Czechoslovakia, RNDr
  • 1991 – 1994  Cancer Research Campaign UK, PhD in Molecular Biology and Radiobiology
  • 1994 - 1997   Research Fellow, Imperial Cancer Research Fund, Clare Hall Laboratories
  • 1997 - 2001   Research Fellow, MRC Cell Mutation Unit, University of Sussex
  • 2001 - 2003   Senior Research Fellow, Genome Damage and Stability Centre, University of Sussex
  • 2003 - 2006   Lecturer, the Medical School, University of Birmingham
  • 2006 - 2018   Senior Lecturer in Human Molecular Genetics, College of Medical and Dental Sciences, University of Birmingham
  • 2018   Senior Lecturer in Human Molecular Genetics, Aston Medical School, Aston Medical Research Institute, Aston University

I lead an active DNA repair and genome stability research that focuses on the area of translational approach in novel neuro-regeneration strategies and nanotechnology mediated drug delivery for cancer treatment. 

  • Manipulation of DNA damage responses to promote neuroregeneration
  • Targeted nanoparticles for experimental cancer therapy
  • The analysis of double-strand break repair within chromatin

 

  • PhD Studentship Kingdom of Saudi Arabia 2017--2021
  • PhD Studentship CARA 2016-2020
  • Dementia Private Fund 2016
  • MRC Confidence in Concept 2013-214                                               
  • Russian Federation PhD studentship 2014                          
  • Gov of Iraq PhD Studentship 2014                                                           
  • EU ESRF project grant 2012                                                                    
  • BWH project grant 2011                                                                
  • EU ESRF project grant 2011                                                                    
  • MRC project grant 2009                                                                
  • EU ESRF project grant 2009                                                                                
  • BCHRF project grant 2009                                                            
  • Egyptian Department of Health PhD Studentship 2007                           
  • MRC/UB PhD Studentship 2006                                                  
  • MRC/EPSRC Discipline Bridging Grants 2006                          
  • MRC/EPSRC Discipline Bridging Grant 2005                          
  • The BBSRC Project Grant, 2005                                                 
  • The Royal Society Project Grant, 2004        
  • Member of the Scientific Grant Review Committee for Medicine at European Union Synchrotron Radiation Facility, Grenoble, France.
  • Member of the Scientific Advisory Review Committee for the Proton Therapy, Ministry of Health, Slovak Republic
  • MRC Career Development Grant Referee
  • MRC Research Grants Referee
  • BBSRC Research Grants Referee
  • FP-7 Intra-European Fellowship Program Referee
  • MRC of Singapore Program Grants Referee
  • INSERM Project Grants Referee

Honorary position: Senior Lecturer in Human Molecular Genetics, College of Medical and Dental Sciences, University of Birmingham

Recent International Conferences (Keynote/invited speaker):

  • 7th International conference on brain and neurological disorders, Amsterdam, Holland April 2018
  • 7th Annual world congress of nanoscience and technology, Fukuoka, Japan 2017
  • 7th European DNA repair workshop, Smolenice 2016
  • London Chromatin Club conference meeting 2016
  • 3rd International Conference on Nanotechnology in Medicine, Manchester, 2015
  • International DNA repair meeting, Smolenice, June 2012
  • International meeting: Nano & Microtechnologies – Medical, Environmental and Electronic Applications, London, October 2010
  • International conference: “Science with X-ray Nano-beams”, Grenoble, February 2010
  • Sixth International DNA repair meeting, Smolenice, May 2008
  • “DNA double-strand break repair in the context of chromatin”, ARR International Meeting, Queens University, Belfast, April 2007
  • “Proteomics in DNA repair” British Council meeting on Genomics and Proteomics of Cancer, Bratislava March 2007 (Also the main organizer invited by the British Council)
  • Fourth International DNA repair meeting on DNA damage and repair: mechanisms and biological consequences, May 2004

Patents:

International Patent: WO2017/199042 Treatment of Neurological pathologies with inhibitors of DNA damage repair (November 2017)

International Patent - WO2013/179014 :

Targeted nanoparticles for cancer imaging and therapy (December 2014, National Phase in EP, US, CN, IN and JP May 2017).

GB Patent: GB1010588.0 Title: Linker Histone H1x (December 2011)

Manuscripts recently submitted:

Richard I. Tuxworth1 #, Matthew J. Taylor1, Ane Martin Anduaga2, Alaa Hussien-Ali3 Sotiroula Chatzimatthaiou1, Joanne Longland4, Adam M. Thompson4, Sharif Almutiri4, Pavlos Alifragis3, Charalambos P. Kyriacou2, Boris Kysela1,5 # and Zubair Ahmed4 #:

Attenuating the DNA damage response to double strand breaks is neuroprotective (# corresponding authors, Nature)

Darren Arbon, Grant Stewart, Christopher Bruce, Karl Nightingale, Mark Jeeves, Michael Overduin and Boris Kysela: A role for the specific linker histone variant in DNA double-strand break repair. (Science).

Sarah Blair-Reid, Christopher Bruce, Darren Arbon, Sylvain Bohic, Stephen P. Hammond, Hellene Elleaume, Zoe Pikramenou and Boris Kysela: Modulation of DNA repair by targeted nanoparticles. (ACS Nano).

Selected published:

Al Emam A, Arbon D, Jeeves M, Kysela B: Ku70 N-terminal lysines acetylation/deacetylation is required for radiation-induced DNA-double strand break repair, Neoplasma, 65(5):708-719,2018.

Murray JE, van der Burg M, IJspeert H, Carroll P, Wu Q, Ochi T, Leitch A, Miller ES, Kysela B, Jawad A, Bottani A, Brancati F, Cappa M, Cormier-Daire V, Deshpande C, Faqeih EA, Graham GE, Ranza E, Blundell TL, Jackson AP, Stewart GS, Bicknell LS.: Mutations in the NHEJ Component XRCC4 Cause Primordial Dwarfism. Am J Hum Genet. 96(3):412-24, 2015

David J. Lewis, Christopher Bruce, Sylvain Bohic, Stephen P. Hammond, Darren Arbon, Sarah Blair-Reid, Zoe Pikramenou and Boris Kysela: Intracellular Synchrotrone X-ray imaging and genotoxicity testing of new luminiscent lanthanide nanobeads. Nanomedicine,10:1547-57, 2011.

Alexandra J. Pope, Christopher Bruce, Boris Kysela, Michael J. Hannon: Issues surrounding standard cytotoxicity testing for assessing activity of non-covalent DNA binding metallo-drugs. Dalton Trans, 39:2772-4, 2010.

Oliver J Brand, Jeffrey C Barrett, Matthew J Simmonds, Paul R Newby, Christopher J McCabe, Christopher K Bruce, Boris Kysela, Jackie D Carr-Smith, Thomas Brix, Penny J Hunt, Wilmar M Wiersinga, Laszlo Hegedus, John Connell, John A H Wass, Jayne A Franklyn, Anthony P Weetman, Joanne M Heward, Stephen C L GoughAssociation of the thyroid stimulating hormone receptor gene (TSHR) with Graves’ disease (GD), Human Molecular Genetics, 2009, 18(9):1704-13.

Robert S. Pitcher, Peter Ahnesorg, Boris Kysela, Chris K. Bruce, Andrew J. Green, Pierre Hentges, Julie Bianchi, Alex Rhie, Stephen P. Jackson and Aidan J. Doherty:

Evolutionary and functional conservation of the DNA non-homologous end-joining protein, XLF/Cernunnos. J Biol Chem. 2006 Dec 8; 281(49):37517-26.

Chovanec, M., Kysela, B*., Dudášová, Z., and A. J. Doherty (2006) DNA non-homologous end-joining in unicellular organisms, in: Valon C. L.

(Ed.), New Developments in Mutation Research, Nova Science Publishers Inc., NY, pp. 149-188. * corresponding author (invited review).

Kysela B, Chovanec M. and Jeggo P.A: Phosphorylation of linker histones by DNA-dependent protein kinase is required for DNA ligase IV-dependent ligation in the presence of histone H1.

Proc Natl Acad Sci U S A. 2005, 8 Feb, 102(6):1877-1882.

Stiff T, Shtivelman E, Jeggo P, and Kysela B.: AHNAK interacts with the DNA ligase IV-Xrcc4 complex and stimulates DNA ligase IV mediated double stranded ligation. DNA Repair, 2004, 4 March, 3(3):245-256.

Girard,P.M*Kysela B*,Riballo E, Harer C,Lobrich M, Doherty A, and Jeggo P.: Structure-function analysis of DNA ligase IV mutations found in LIG4 syndrome patients: impact of two linked polymorphismsHuman Molecular Genetics, 2004, 13(20): 1-8,  * first authors

Kysela B, Doherty AJ, Chovanec M, Stiff T and Jeggo PA.: Ku stimulation of DNA ligase IV-dependent ligation requires inward movement along the DNA molecule. J  Biol Chem, 2003 Jun 20;278(25):22466-74.

Smith J, Riballo E, Kysela B, Baldeyron C, Manolis K,Masson C, Lieber MR, Papadopoulo D, Jeggo P.: Impact of DNA ligase IV on the fidelity of end joining in human cells, Nucleic Acid Research, 2003, Apr 15, 31(8):2157-67.

Dai Y, Kysela B, Manolis K, Riballo E, Harwille T, Jeggo PA, Oettinger M.: A human SCID cell line demonstrates that an additional factor is required for non-homologous end-joining and V(D)J recombination. Proc Natl Acad Sci USA. 2003, Mar 4, 100(5):2462-7.

Kuniyoshi Iwabuchi, Balaku P. Basu, Boris Kysela Takayuki Kurihara, Masao Shibata, Deyu Guan, Yongheng Cao, Tomio Hamada, Junya Kobayashi, Hiroshi Tauchi, Hidetoshi Aoki, Noboru Motoyama, Kenshi Komatsu, Kouji Imamura Penny A. Jeggo, Takayasu Date, Aidan J. Doherty:p53-binding protein 1 (53BP1) binds to DNA at IR damage-induced foci and promotes DNA double-strand break repair, J Biol Chem, 2003, 278:36487-95. (2003).

Weller GR* Kysela B*, Roy R* Tonkin LM, Scanlan E, Della M, Devine SK, Day JP, Wilkinson A, di Fagagna F, Devine KM, Bowater RP, Jeggo PA, Jackson SP, Doherty AJ.: Identification of a DNA nonhomologous end-joining complex in bacteria. Science2002 Sep 6;297(5587):1686-9. * first authors

O'Driscoll M, Cerosaletti KM, Girard PM, Dai Y, Stumm M, Kysela  B, Hirsch B, Gennery A, Palmer SE, Seidel J, Gatti RA, Varon R, Oettinger MA, Neitzel H, Jeggo PA, Concannon P.: DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency. Mol Cell. 2001 Dec;8(6):1175-85

Riballo E, Doherty AJ, Dai Y, Stiff T, Oettinger MA, Jeggo PA and Kysela B.: Cellular and biochemical impact of a mutation in DNA ligase IV conferring clinical radiosensitivity. J Biol Chem. 2001 August 17; 276 (33): 31124-32.

Beamish HJ, Jessberger R, Riballo E, Priestley A, Blunt T, Kysela B, Jeggo PA.The C-terminal conserved domain of DNA-PKcs, missing in the SCID mouse, is required for kinase activity. Nucleic Acids Res. 2000 Apr 1;28(7):1506-13.

Riballo E, Critchlow SE, Teo SH, Doherty AJ, Priestley A, Broughton B, Kysela B, Beamish H, Plowman N, Arlett CF, Lehmann AR, Jackson SP, Jeggo PA.: Identification of a defect in DNA ligase IV in a radiosensitive leukaemia patient. Curr Biol. 1999 Jul 1;9(13):699-702.

Kysela B., Lohrer H. Arrand JE: Defects in the kinetics of the repair of DNA double-strand breaks and inhibition of DNA synthesis in the ataxia telangiectasia AT5BI-VA cell line: comparison to a corrected hybrid, atxbc, Radiat Res. 1995, 144(3):276-81

Kysela B, Michael BD, Arrand JE: Relative contributions of lelvel of initial damage and repair of double-strand breaks to the ionizing radiation-sensitive phenotype of the Chinese hamster cell mutant, XR-V-15B: part I. X-rays. Int J Radiat Biol. 1993; 63:609-16.

Kysela B, Arrand JE, Michael BD: Relative contributions of lelvel of initial damage and repair of double-strand breaks to the ionizing radiation-sensitive phenotype of the Chinese hamster cell mutant, XR-V-15B: part II. Neutrons. Int J Radiat Biol. 1993; 64:531-8.

Kysela B, Michael BD, Arrand JE: Field-inversion gel electrophoresis analysis of the induction and rejoining of DNA double-strand breaks in cells embedded in agarose. Radiat Res. 1993, 134:107-11.

Arrand JE and Kysela B.: Molecular analysis of radiation damage and repair in normal and mutant cells. British Journal of Radiology 1992; 24:43-7.